Qalab cusub oo Coronavirus ah (SARS-Cov-2) Qalabka Ogaanshaha Asiidhka Nucleic

New Coronavirus(SARS-Cov-2) Nucleic Acid Detection Kit

(Flumacdanscent RT-PCR Probe Method) Product Manual

【Product name 】Xirmada Garashada Asiidhka Nucleic-ka Cusub (Habka Baarista Fluorescent RT-PCR)

【Packaging specifications 】25 Tijaabooyin/Qab

【Intended usda'da】

Qalabkan waxaa loo isticmaalaa in lagu ogaado tayada nucleic acid ee ka timaada coronavirus-ka cusub ee sanka sanka, suufka oropharyngeal (cunaha), suufka sanka hore, suufka dhexe ee turbinate, sanka dhaqida iyo damacyada sanka ee shakhsiyaadka looga shakisan yahay COVID19 bixiyehooda daryeel caafimaad. Helitaanka hiddo-wadaha ORF1ab iyo N ee coronavirus-ka cusub ayaa loo isticmaali karaa ogaanshaha kaaliyaha iyo la socodka cudurrada faafa ee caabuqa cusub ee coronavirus.

【Principles of the prosdure 】

Xirmadan waxaa loogu talagalay baaritaanno gaar ah oo TaqMan ah iyo aasaasiyaal gaar ah oo loogu talagalay coronavirus-ga cusub (SARS-Cov-2) ORF1ab iyo taxanaha hiddo-wadaha N. Xalka falcelinta PCR-ga waxa uu ka kooban yahay 3 qaybood oo ah aasaasayaal gaar ah iyo shaybaarro florescent ah oo lagu ogaanayo bartilmaameedyada gaarka ah, iyo qayb dheeraad ah oo ka mid ah furayaasha gaarka ah iyo baarayaasha fluorescent ayaa loo isticmaalaa xakamaynta heerka gudaha ee xirmada si loo ogaado hiddo-wadaha ilaalinta guryaha.

Mabda'a tijaabada ayaa ah in baaritaanka fluorescent ee gaarka ah la dheefshiido oo hoos u dhigo waxqabadka exonuclease ee Taq enzyme ee falcelinta PCR, si kooxda fluorescent weriyaha iyo kooxda fluorescent la demiyo la kala saaro, si nidaamka kormeerka fluorescence uu u helo fluorescent. calaamada, ka dibna Iyada oo loo marayo saamaynta kobcinta PCR-weynaynta, calaamada fluorescence ee baadhitaanku waxa ay gaartaa heerka ugu go'an ee qiimaha-Ct (Cyclethreshold). Haddii ay dhacdo in aan amplicon bartilmaameed lahayn, kooxda weriyaha ee baarayaashu waxay ku dhow yihiin kooxda deminta. Waqtigan xaadirka ah, wareejinta tamarta resonance resonance waxay dhacdaa, iyo fluorescence kooxda weriyeyaasha waxaa demiyay kooxda demisay, si calaamada fluorescent aan lagu ogaan karin qalabka PCR ee fluorescent.

Si loola socdo isticmaalka reagents inta lagu jiro tijaabada, xirmada waxaa lagu qalabeeyaa kontaroolo togan iyo kuwa taban: kantaroolka togan wuxuu ka kooban yahay goobta la beegsanayo ee plasmid recombinant, iyo kantaroolka taban waa biyo Distilled, kaas oo loo isticmaalo si loola socdo wasakhowga deegaanka. Waxa lagu talinayaa in la sameeyo xakamayn togan iyo xakamayn taban isku mar marka la tijaabinayo.

【Main components 】

Ogow：(1) Qaybaha xirmooyinka dufcadaha kala duwan lama isku dari karo lamana beddeli karo.

(2) Diyaarso reagen kuu gaar ah: Xidhmada soo saarista aashitada nukliyeerka.

【Storage conditions iyo expiration date 】

For BST-SARS-25:Gaadiidka iyo kaydinta -20±5℃ waqti dheer.

For BST-SARS-DR-25:Gaadiidka heerkulka qolka. Ku kaydi at -20±5℃ waqti dheer.

Iska ilaali wareegyada qaboojinta ee soo noqnoqda. Muddada ansaxinta si ku meel gaar ah ayaa loo dejiyay 12 bilood.

Fiiri calaamadda taariikhda la sameeyay iyo isticmaalka.

Furitaanka ugu horreeya ka dib, reagent waxaa lagu kaydin karaa -20 ± 5 ° C wax aan ka badnayn 1 bil ama ilaa dhammaadka muddada reagent, taariikhda kasta oo soo horraysa, si looga fogaado soo noqnoqda wareegyada qaboojinta, iyo tirada reagent baraf. - wareegyada dhalaaliddu waa in aanay dhaafin 6 jeer.

【Applicable instrument】ABI 7500, SLAN-96P, Roche-LightCycler-480.

【Sample requirements 】

1. Nooca muunada lagu dabaqi karo: Xalka aashitada nucleic ee la soo saaray.

2.Sample Kaydinta iyo Gaadiidka: Ku kaydi at-20±5℃ muddo 6 bilood ah. Qabo oo dhalaali shaybaarada wax ka badan 6 jeer.

【Testing method】

1.Nucleic acid extraction

Dooro xirmo ku habboon soo saarista aashitada nucleic si aad u soo saarto fayras nucleic acid, oo raac tilmaamaha xirmada u dhigma. Waxaa lagu talinayaa in la isticmaalo xirmada soo saarista aashitada nucleic-ka iyo xirmada sifaynta ee ay soo saartay Yixin Bio-Tech (Guangzhou) Co., Ltd.

2.  Reaction dib u habaynnt preparation

2.1 For BST-SARS-25:

(1) Ka saar SARS-Cov-2 Mix iyo Enzyme Mix, si buuxda ugu dhalaali heerkulka qolka si fiican u walaaq qalabka Vortex ka dibna si kooban u qabooji.

(2) 16.5uL Enzyme Mix ayaa lagu daray 358.5uL SARS-Cov-2 Isku qas oo si fiican loo qaso si loo helo xalka falcelinta isku dhafan.

(3) Diyaari tuubo octal PCR 0.2 mL nadiif ah oo ku calaamadee 15ul ee xalka falcelinta isku dhafan ee kor ku xusan midkiiba.

(4) Ku dar 15 μL oo ah dareeraha aashitada nucleic ee la safeeyey, xakamaynta togan iyo xakamaynta taban, oo si taxadar leh u dabool daboolka tuubada octal.

(5) Si fiican isku qas adigoo kor u rogaya, oo si degdeg ah u qabooji si aad isugu ururiso dareeraha hoose ee tuubada.

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