Xirmada Soosaarka Asiidhka Nukliyeerka ah
Nkleic Acid Esoo jiidasho Or Nadiifinta Kitama lagu kaydiyaa -20 ℃. Muunada waa in lagu raro iyada oo la isticmaalayo 0℃ curling.
Introduction
Qalabka Soosaarka Asiidhka Nucleic-ka ama Qalabka Sifaynta (Habka Biraha Biraha) waxaa loogu talagalay in si toos ah loo sifeeyo RNA iyo DNA laga soo qaado dareerayaasha jirka (sida suufka, balaasmaha, serum) iyadoo la isticmaalayo qalabyada soo saarista aashitada nucleic ee otomaatiga ah. Farsamaynta qayb-magnetik-ku waxay bixisaa DNA/RNA tayo sare leh oo ku habboon in si toos ah loogu isticmaalo codsiyada hoose sida xoojinta ama falcelinta enzymatic kale.
Application Range
Dhiigga oo dhan, balaasmaha, serum iyo shaybaarada unugyada ayaa si toos ah loo jeexjeexay oo la dheefshiiday. Aashitada nucleic-ka ah ee la sii daayay waxaa si xushmad leh u galiyay super paramagnetic nanometer magnetic. Kadibna borotiinka, iiyooyinka milixda aan organic iyo wasakhda organic ayaa laga saaray xal dhaqid.Ugu dambeyntii, aashitada nucleic-ka ayaa la soo saaray si loo helo xal nucleic ah oo saafi ah.
Kit Contents
| Bisad Maya | YXN-VIRAL01-32A-BR | Qaybaha | ||
| -50A | - 100A | |||
| Cabbirka | 32 Tes | 50Tijaabi | 100 imtixaan | |
| Lysis Buffer | 96 ceel-baakad ed Taarikada 2 qaybood | 25ml | 50ml | Surfactant iyo Tris |
| Wash Buffer I | ★15ml | ★30ml | Xalka milix badan | |
| Wash Buffer Il | ★6ml*2 | ★12ml*2 | Xalka milix-yar | |
| Elution Buffer | 10ml | 20ml | Xalka milix-yar | |
| MagaBio Reagent | 1.0ml | 2.0ml | Qaybaha magnetic | |
| Buug-gacmeed (=YXN-VIRAL01-32A-BR) | 1 | 1 | 1 | |
| Notes:WaayoYXN-VIRAL01-32A-BR-50A,ku dar 15mL Absolute ethanol ★15mL Wash Buffer I ka hor isticmaalka; ku dar 24mL Absolute ethanol to ★6mL Ku dhaq bakhaarka Il ka hor isticmaalka. | ||||
| WaayoYXN-VIRAL01-32A-BR-100A, ku dar 30mL Absolute ethanol ★30mL Wash Buffer I ka hor isticmaalka; ku dar 48mL Absolute ethanol ★12mL Wash baffer Il ka hor intaan la isticmaalin.【Reagents uu diyaariyo isticmaaluhu | ||||
Storage Conditions
Marka qalabka la yimaaddo, qaybaha qalabka waxa lagu kaydin karaa heerkulka qolka (15 - 25°C). Dib-u-hawlgalayaashu waxay xasilloon yihiin illaa hal sano laga bilaabo taariikhda wax-soo-saarka.
Sample Requirements
1. Muunad lagu dabaqi karo: suufka, balaasmaha, serum iyo dhiiga oo dhan iwm.
2. Tusaalaha kaydinta iyo gaadiidka: Muunada waa in isla markiiba la tijaabiyaa
Materials iyo Devices Required but Not Provided
1. galoofyada aan budada lahayn ee la tuuri karo
2. Weelka halista nafleyda
3. Qalin ama per
Procedure
Kuwan soo socdaa waxay adeegsadaan muunadda xariijimaha soo saarista swab labeen tusaale ahaan si ay si kooban ugu sharaxaan tillaabooyinka hawlgalka reagent soo saarista aaladda soo saarista aashitada bayoolojiBioer NPA-32PamaSMFANKA 32. Noocyada muunadyada kale, fadlan tixraac buug-gacmeedka isticmaale. Waxa kale oo ay ku shaqayn karaan macaamiishu iyadoo loo eegayo helitaanka tijaabada ah:
1. Reagent Diyaarinta
a. WaayoYXN-VB03-32A-50A iyo YXN-VB03-32A-100A
Ku dar 500uL Lysis Buffer tiirka 1 iyo 7 ee 2.2mL 96 saxan ceel-qoto dheer, 500uL Wash Buffer I tiirka 2 iyo 8, 500uL Wash Buffer II tiirka 3, 4 iyo 9,10; 70uL Elution Buffer ilaa tiirka 5 iyo 11, 180uL biyo saafi ah iyo 20uL MagaBio Reagent ilaa tiirka 6 iyo 12
b. WaayoYXN-VB03-32A
Ku rid 96 reagents oo si wanaagsan loo diyaariyey heerkulka qolka. Saxanka 96-si fiican u rux kor ilaa saddex jeer, oo ka jeex bacda caagga ah. Centrifuge reagement hore loo sii diyaariyey dhawr ilbiriqsi (ama gacanta ku lullaabo dhawr jeer) si aad uga fogaato reagenti ku dheggan gidaarka tuubooyinka. Jeex filimka aluminiumka ah ee saxan 96-ceel ah oo cadee jihada saxanka (kuulo birlab ah oo ku jira tiirka #6 iyo #12),
2.Tusaale Soo saarid
1. Ku dar muunada 300uL ee 96 tiirarka saxan ee ceelka #1 iyo #7, fadlan ka fogow faddaraynta.
2. Dhig saxan ceel qoto dheer oo 96 ah oo ku dheji qalabka, ku dheji talooyinka 8-strip ee qalabka,
3. Ku socodsii barnaamijka si waafaqsan nidaamka soo socda.
4. Kadib marka nadiifinta tooska ah ay dhamaato, u wareeji Buffer Buffer ee tiirarka 5 iyo 11 una wareeji tuubo centrifuge nadiif ah oo ah 0.5mL; Haddii aanad isla markiiba isticmaalin, fadlan ku kaydi heerkulka -20 °C.
Performance Characteristics
1. Alaabta la soo saaray waxaa lagu ogaadaa dareenka sare ee HBV DNA reagent si loo gaaro dareenka 10 IU/ml. Alaabta la soo saaray waxaa lagu ogaadaa dareenka sare ee HCV RNA reagent ogaanshaha si loo gaaro dareenka 50 IU/ml.
2. Dooro 4 muunadood (muunada serum/plasma, muunada sanbabada sanbabada, muunada unugga exfoliated ee afka ilmagaleenka), muunad kasta waxaa lagu qasi karaa 10 jeer 3 gradients (oo ay ku jiraan muunada asalka ah ee wadarta 4 uruurinta), iyadoo la isticmaalayo reagents iyo wakiilada imtixaanada si loo ogaado gudaha hiddo-wadaha tixraaca sida waafaqsan tilmaamaha badeecada, iyo qiimaha Ct ee dufcad kastaa way ku kala duwan tahay wax ka yar 1.
| Step | Waa hagaag Goobta | Program Magaca | Waiting Time(min:SS) | Mixing Time(mgudaha:SS) | Magnet Time(min:SS) | Afoolxumo | Sdhidid | Volume tatus(μL) | Temperature |
| 1 | 1 | Lysis | 0:00 | 2:00 | 0:00 | F | 700 | 80 | |
| 2 | 6 | Bdhegaha | 0:00 | 0:15 | 0:15 | √ | F | 200 | |
| 3 | 1 | Bind | 0:00 | 3:00 | 0:45 | √ | F | 700 | |
| 4 | 2 | Wdambas1 | 0:00 | 0:30 | 0:30 | √ | F | 500 | |
| 5 | 3 | Wdambas2 | 0:00 | 0:30 | 0:30 | √ | F | 500 | |
| 6 | 4 | Wdambas3 | 0:00 | 0:30 | 0:30 | √ | F | 500 | |
| 7 | 5 | Ilbaxnimo | 2:00 | 2:30 | 0:30 | F | 70 | 80 | |
| 8 | 6 | Tuur | 0:00 | 0:15 | 0:00 | F | 200 |
Safety
1. GUUDAL BADBAADADA.
Isticmaalka alaabtan si aan lagu cayimin dukumeenti isticmaaluhu waxay keeni kartaa dhaawac shakhsi ah ama dhaawac soo gaara qalabka ama qalabka. Hubi in qof kasta oo isticmaalaya alaabtan uu helay tilmaamo ku saabsan dhaqamada badbaadada guud ee shaybaarada iyo macluumaadka badbaadada ee lagu bixiyay dukumeentigan.
1.1 Kahor intaadan isticmaalin qalab ama qalab, akhri oo fahan macluumaadka badbaadada ee lagu bixiyay dukumeenti isticmaale ee uu bixiyay soo saaraha qalabka ama aaladda.
1.2 Kahor intaadan la tacaalin kiimikooyinka, akhri oo fahan dhammaan xaashida Xogta Badbaadada ee khuseeya (SDSs) isticmaalna qalabka ilaalinta shakhsi ahaaneed ee habboon ( galoofyada, gogosha, ilaalinta indhaha, iwm.). Si aad u hesho SDS, eeg qaybta "Dokumentiyada iyo Taageerada" ee dukumeentigan.
2. Kiimiko badbaadada
MAAMULKA GUUD EE KIIMIKADA. Si loo yareeyo khataraha, hubi in shaqaalaha shaybaadhka akhriyaan oo ay ku dhaqmaan tilmaamaha guud ee badbaadada isticmaalka kiimikada, kaydinta, iyo qashinka ee hoos ku qoran, oo kala tasho SDS ku habboon taxaddar iyo tilmaamo gaar ah: Akhri oo fahma xaashida Xogta Badbaadada (SDSs) ee ay bixiso kiimikaad soo saaraha ka hor inta aanad kaydin, gacanta ku dhigin, ama ku shaqayn wax kiimiko ah ama walxo khatar ah.
2.1 Yaree xidhiidhka kiimikooyinka. Xiro qalabka ilaalinta shakhsi ahaaneed ee habboon marka la qabanayo kiimikooyinka (tusaale, muraayadaha badbaadada, galoofyada, ama dharka ilaalinta).
2.2 Yaree neefsashada kiimikooyinka. Ha ka tagin weelasha kiimikada oo furan. Isticmaal kaliya hawo ku filan (tusaale, daboolka qiiqa).
2.3 Si joogto ah u hubi in ay kiimiko ka daadato ama daadato. Haddii daadato ama daadato ay dhacdo, raac nidaamka nadiifinta warshadeeyaha sida lagu taliyay SDS.
2.4 Ku xakamee qashinka kiimikaad ee daboolka qiiqa.
2.5 Hubi isticmaalka weelasha qashinka aasaasiga ah iyo kuwa sare. (Qashinka aasaasiga ah
weelka ayaa haya qashinka degdega ah. Weelka labaad wuxuu ka kooban yahay daadad ama dheecaan ka yimaadda weelka aasaasiga ah. Labada weelba waa inay la jaanqaadaan walaxda qashinka oo ay buuxiyaan shuruudaha federaalka, gobolka, iyo deegaanka ee kaydinta weelka.).
2.6 Ka dib marka aad faaruqiso weelka qashinka, ku xidh daboolka la dhigay.
2.7 Ku sifoow (falanqaynta haddii loo baahdo) qashinka ay soo saaraan codsiyada gaarka ah, reagents, iyo substrates ee lagu isticmaalo shaybaadhkaaga.
2.8 Hubi in qashinka la kaydiyo, la wareejiyo, la raro, lana tuuro si waafaqsan dhammaan xeerarka deegaanka, gobolka/gobolka, iyo/ama qaranka.
2.9 Walxaha shucaaca ah ama noolehazardous waxay u baahan karaan maarayn gaar ah, waxaana la adeegsan karaa xaddidaadyo tuurista.
3.Bayooloji khatar badbaadada
Biyohazard suurtagal ah. Iyada oo ku xidhan muunadaha loo isticmaalo qalabkan, dusha sare waxa loo qaadan karaa halis noole. Isticmaal hababka sun-saarida habboon marka aad la shaqaynayso khataraha noolaha.
BIOHAZARD. Tijaabooyin bayooloji ah sida unugyo, dheecaannada jidhka, jeermisyada faafa, iyo dhiigga dadka iyo xayawaanka kale waxay awood u leeyihiin inay gudbiyaan cudurrada faafa. Raac dhammaan xeerarka deegaanka, gobolka/gobolka, iyo/ama qaranka ee khuseeya. Xiro qalabka ilaalinta habboon, oo ay ku jiraan laakiin aan ku xaddidnayn: muraayadaha ilaalinta, gaashaanka wejiga, dharka/jaakada shaybaadhka, iyo galoofyada. Dhammaan shaqada waa in lagu qabtaa tas-hiilaad si habboon u qalabaysan iyadoo la isticmaalayo qalabka badbaadada ee ku habboon (tusaale, aaladaha xakamaynta jirka). Shakhsiyaadka waa in loo tababaraa si waafaqsan sharciyeynta iyo shuruudaha shirkadda/machadka ka hor inta aan lala shaqayn agabka faafin kara.
Akhri oo raac tilmaamaha lagu dabaqi karo iyo/ama shuruudaha sharci ee soo socda:
Gudaha Maraykanka: Tilmaamaha Waaxda Caafimaadka iyo Adeegyada Aadanaha ee Maraykanka oo lagu daabacay Biosafety ee Shaybaarada Microbiological iyo Biomedical laga helay: www.cdc.gov/biosafety.
Heerarka Badbaadada Shaqada iyo Caafimaadka, Cudurada Dhiiga Ka Dhashay (29 CFR§1910.1030), laga helay:
www.access.gpo.gov/nara/cfr/waisidx_01/29cfr1910a_01.html
borotokoollada Barnaamijka Biosafety ee shirkaddaada/machadka ee ka shaqaynta/maaraynta walxaha suurtagalka ah ee faafa. Macluumaad dheeraad ah oo ku saabsan tilmaamaha biohazard ayaa laga heli karaa: www.cdc.gov.
Gudaha Midowga Yurub: Hubi xeerarka deegaanka iyo sharciga ilaalinta biohazard iyo badbaadada noolaha oo tixraac dhaqamada ugu wanaagsan ee lagu daabacay Ururka Caafimaadka Adduunka (WHO) Buugga Badbaadada Nafleyda, daabacaadda saddexaad, laga helay: www.who.int/csr/resources/publications / biosafety/WHO_CDS_CSR_LYO_200 4_11/m/.
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